Authors : Jianlong Wang, Xiaozhe Zhou, Xiaowei Xing, Yanliang Chen, Gangwen Guo, Jianfeng Sun, Qingshan Zhang and Minren Shen
Abstract: Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family of growth factors and plays significant role in central nervous system and the peripheral nervous system for supporting the survival of existing neurons and encouraging the growth and differentiation of new neurons and synapses. Increasing study indicate that BDNF is associated with neurological disorders and psychiatric diseases. Beyond the importance of BDNF in nervous system, compelling evidence also demonstrate that it is also essential for body weight control and energy homeostasis. BDNF therefore, become the target in various diseases research such as Alzheimer’s disease, depressive disorder and obesity. In this study, researchers cloned the BDNF gene to p-EGFP N1 expression plasmid, meanwhile, researchers also transfected the p-EGFP N1-BDNF to Hela cell with lipofection. Fluorescence microscope and Western blot analysis showed BDNF could normally express in Hela cell. However, the physiological functions and pharmacological roles of the p-EGFP N1-BDNF need further validation in cellular level and animal model.
Jianlong Wang, Xiaozhe Zhou, Xiaowei Xing, Yanliang Chen, Gangwen Guo, Jianfeng Sun, Qingshan Zhang and Minren Shen, 2012. Construction and Transfection of EGFP-N1-BDNF Expression Plasmid. Journal of Animal and Veterinary Advances, 11: 4599-4602.