Authors : Hua-Li Wu, Hui-Ying Wang, Da-Qian He, Yi Liu, Shao-Ming Gong, Fan-Li Kong and Qing Zhu
Abstract: Peroxisome Proliferator-Activated Receptor γ (PPAR-γ) was a key regulator of proliferation and preadipocyte differentiation in mammals. The objective of this study was to investigate the effect of the PPAR-γ gene on slaughter traits of 170 individuals from Zhejiang White geese (ZW) and the ontogenetic expression pattern. PCR-SSCP technique was developed to analyze coding region of the PPAR-γ gene polymorphism. The results showed that two genotypes (AA and AG) which were the products of two alleles (A and G) were observed. Association analysis indicated that this SNP was significantly associated with percentage of goose leg muscle (p<0.05) and the allele A was the predominant allele. Quantitative real-time PCR (qRT-PCR) assay measured the PPAR-γ mRNA expression in ZW and Landes goose five tissues of different ages (P1, P14, P21, P28, P35, P42, P49, P56 and P63). The results showed that the PPAR-γ mRNA had the highest expression level in goose abdominal fat and subcutaneous fat followed by lung and intestine. The PPAR-γ mRNA levels exhibited a rise-decline change in fat tissues except for ZW subcutaneous fat. The values in fat tissues were higher than those of other tissues at the same goose breed (p<0.01). Besides, average values in ZW fat and liver tissues were higher than those of Landes goose totally. These results suggested that the PPAR-γ expressions were positively associated with goose fat development and had a breed-related tendency. The PPAR-γ gene polymorphism could be used in Marker Assistant Selection (MAS) as a genetic marker for goose slaughter traits.
Hua-Li Wu, Hui-Ying Wang, Da-Qian He, Yi Liu, Shao-Ming Gong, Fan-Li Kong and Qing Zhu, 2012. Ontogenic Expression Pattern and Genetic Polymorphism of the Peroxisome Proliferator-Activated Receptor (PPAR-γ) Gene in Goose Populations. Journal of Animal and Veterinary Advances, 11: 4625-4631.