Authors : Francisco Tobias Barradas-Pina, David Itzcoatl Martinez-Herrera, Alvaro Enrique de Jesus Peniche-Cardena, Ruben Dario Serna-Montero, Francisco Morales-Alvarez, Ricardo Flores-Castro, Ruben Loeza-Limon and Maria Luisa Robledo-Salinas
Abstract: With the aim of measuring preescapular lymph node response in bovines vaccinated with either S19 or RB51 Brucella abortus strains vaccines, two herds were selected. About 40 females were divided in two groups of 20; group 1 was vaccinated and group 2 left as unvaccinated control. Vaccinated group, 3 and 6 months females were vaccinated with 5x1010 Colony Forming Units (CFU) dosage and >6 with a 3x108-3x109 S19 strain. For the second herd same age females and dosage was used but applying RB51 strain. Vaccines were subcutaneously applied at mid third on left side of the neck. Preescapular lymph nodes were measured in length and wide at 90 and 180 days after vaccination. Results were analyzed by Chi-square. At 90 days post vaccine, lymph nodes dimensions were 3.7-4.8 cm widex10-11.7 cm length and for unvaccinated 3.8-4.5x10-11.5 cm. No statistical differences were found (p>0.05) among vaccinated and unvaccinated controls. After 180 days post vaccine similar results were found with RB51 for both groups but strain 19 vaccinated herd was different (p<0.05) however, differences were found in control group. Results indicate that both strains are efficiently processed by lymphatic tissue among 90 days after vaccination and therefore, they may be considered as safe without producing macroscopic changes that alter organ dimensions.
Francisco Tobias Barradas-Pina, David Itzcoatl Martinez-Herrera, Alvaro Enrique de Jesus Peniche-Cardena, Ruben Dario Serna-Montero, Francisco Morales-Alvarez, Ricardo Flores-Castro, Ruben Loeza-Limon and Maria Luisa Robledo-Salinas, 2012. Morpho Metrical Evaluation of Preescapular Lymph Nodes from Cattle Vaccinated with Brucella abortus Strains S19 or RB51. Journal of Animal and Veterinary Advances, 11: 521-525.