Abstract: One of the real problems that cause the public health risks in small animal yearly is parasitic disease and some of these parasites are zoo noses. To overcome these problems the use of antiparasitic drugs is necessary. Ivermectin is a broad spectrum antiparasitic agent and different routes of its administration such as injection, oral and pour-on were used. The aims of the current study were evaluation of the efficacy of Ivermectin pour-on administration against natural Ancylostoma caninum infection in native dog and also determination the prevalence rate of this parasite in East-Azerbaijan province, Iran. In the present study, 60 native dogs were investigated by Egg Per Gram of feces (EPG) feces test. Willis method was applied for feces test and Mack-Master slide method was used for counting nematode eggs. After confirming the infection with worms, Ivermectin (0.5 mg kg^-1) pour-on was administrated to infected dogs. Fecal examination was repeated in 1, 7, 21 and 28 days post treatment. Results showed that total prevalence of Ancylostoma caninum infection was 28.33% in native dog of East-Azerbaijan province, Iran. Efficacy rate of Ivermectin pour-on was 21.19, 68.21, 91.24 and 98.61% in 1, 7, 21 and 28 days, respectively. In conclusion, the effect of this drug against Ancylostoma caninum resulted in reduction in egg count exceeded 98% so, this drug can be used in antiparasitic program in dog. Further investigations are necessary to evaluate the drug effect on other nematodes and parasitic infections.