Abstract: To investigate anti-Neospora caninum antibodies in stray dogs living in Tabriz city, 100 blood samples were collected. Serum samples were screened for detection of anti-N. caninum IgG antibodies using Indirect Fluorescent Antibody Test (IFAT; ≥50). Antibodies were seen in 31 (31%) of 100 dogs. The IFAT antibody titers were as follows: 1:50 in 11 dogs, 1:100 in 10 dogs, 1:200 in 6 dogs, 1:400 in 1 dog, 1:800 in 2 dogs and 1:1,600 in 1 dog. There were no significant differences in seroprevalence of Neospora infection between different genders (p>0.05). The seropositive results were increased with age and the differences were statistically significant (p<0.05). The results confirm the presence and exposure of stray dogs to N. caninum in Tabriz city and the importance of this protozoan as a cause of disease in dogs of the region.