Abstract: MMPs are vital in menstruation. This study aimed to investigate their expressions in a mouse menstrual-like model by pharmacologic progesterone withdrawal. The model was prepared by administration of mifepristone after decidualization of ovariectomized mice. Immunohistochemistry was performed to detect the locations of Matrix Metalloproteinase (MMPs) proteins. MMP3 was present in the focal subluminal stromal zone, epithelium, decidual zone and interface between the basal zone and functional zone from 0-24 h and epithelium and subluminal epithelium stroma from 32-48 h. MMP7 was present in the basal zone, epithelium and mid decidual zone from 0-24 h and a similar location to MMP3 from 32-48 h. MMP9 was present in the basal zone and focal zone of the subluminal stroma from 0-24 h, epithelium and the edge of tissue breakdown at 32 h, basal zone and the subluminal stroma at 40 h and the subluminal stroma at 48 h. MMP13 was present in the mid decidual zone at 24 and 32 h and epithelium at 40 and 48 h. MMP2 was present in the basal zone and mid decidual zone from 0-24 h, the mid decidual zone at 32 h and no staining at 40 and 48 h. The locations of MMPs protein in the model displayed regional character and showed consistency with those in the mice model by physiological progesterone withdrawal and in primates.
Xiangbo Xu, Xihua Chen, Jiedong Wang and Bin He, 2013. Matrix Metalloproteinase Expression in a Mouse Menstrual-Like Model by Pharmacologic Progesterone Withdrawal. Journal of Animal and Veterinary Advances, 12: 1139-1146.