Authors : Kun Wang, XiaoTing Chen, RongWei Zhao, QiRui Hao, QiRui Hao, GeFeng Xu, XuYang Jiang and Ying Han
Abstract: Researchers cloned the Leptin of Culter alburnus in Xingkai Lake and analyzed the sequence characteristic of Leptin coding protein and detected the relative expression levels of Leptin RNA in wild and cultured Culter alburnus in Xingkai Lake of different ages. The result provide reference on studying the regulatory effect and molecular mechanism of Leptin on food intake, digestion and catabolism genes and in Culter alburnus in Xingkai Lake. The full length cDNA of Leptin gene was got by RT-PCR, 5'-RACE and 3'-RACE. The primer of semi-quantitative RT-PCR and real-time PCR were designed by the acquired cDNA then the gene expression was studied. The full sequence length of Leptin in Culter alburnus in Xingkai Lake was 1208 bp, the ORF length is 522 bp which code 173 amino acids. With the increase of age, the expression of mRNA in both wild and cultured Culter alburnus in Xingkai Lake show rising trend, from 3 years old, the expression of Leptin in wild population showed down regulation in contrast to the cultured population.
Kun Wang, XiaoTing Chen, RongWei Zhao, QiRui Hao, QiRui Hao, GeFeng Xu, XuYang Jiang and Ying Han, 2013. Cloning and Analysis of Leptin in Culter alburnus in Xingkai Lake and down Regulating its Expression Compared to Cultured Population. Journal of Animal and Veterinary Advances, 12: 128-134.