Authors : Jian-Li Shi, Xiao-Yan Cong, Xiao-Yuan Yuan, Jia-Qiang Wu, Wen-Bo Sun, Yi-Jun Du, Zhe Peng, Jun Li, Jin-Bao Wang, Bao-Hua Huang and Dai-Cheng Liu
Abstract: Porcine Circovirus type 2 (PCV2), the essential causative agent of the Post weaning Multisystemic Wasting Syndrome (PMWS) has posed a grave threat to global swine industry in recent decades. Infectious molecular clone is the best way to get the purificated virus for pathogenesis study and inactivated vaccine produce. The purpose of this study was to compare and get the best way for PCV2 infectious molecular clone to rescue viruses. In this study, four PCV2 infectious molecular clone was constructed and transfected into PK-15 cells. IFA and Real-time PCR was used to detect the presence of PCV2. The IFA results revealed the precence of rescued viruses. The real-time PCR results showed that there were the most DNA copies with Ligation DNA transfection, the least DNA copies with pEASY-PCV2 transfection and almost the same with psk-2PCV2 and psk-PCV2 transfection. This assay afford the best way to construct and get PCV2 infectious molecular clone for further research.
Jian-Li Shi, Xiao-Yan Cong, Xiao-Yuan Yuan, Jia-Qiang Wu, Wen-Bo Sun, Yi-Jun Du, Zhe Peng, Jun Li, Jin-Bao Wang, Bao-Hua Huang and Dai-Cheng Liu, 2013. Methods Compare for Rescued Porcine Circovirus Type 2 Viruses. Journal of Animal and Veterinary Advances, 12: 1296-1298.