Authors : Zhao Li, Jie Gao, Pengji Gao, Wenxia Li and Jiye Zhu
Abstract: To investigate the effects of recombinant Growth Hormone (rGH) preconditioning on rat liver ischemia-reperfusion injury. After rat hepatic ischemia-reperfusion model was established, animals were divided into experimental group, control group and sham group (n = 10 each group). The experimental group was subcutaneously injected with rGH (1 U/kg/day) for 1 week while the control group was given the same volume of saline. Perfusion was recovered at 40 min after portal triad clamping in both experimental and control groups. The serum levels of Tumor Necrosis Factor α (TNF-α) and Interleukin 1β (IL-1β) and Super Oxygen Dehydrogenises (SOD) and Methane Dicarboxylic Aldehyde (MDA) in liver homogenate were examined 2 and 24 h after reperfusion. Liver ultrastructural changes were observed under electron microscopy and Apoptosis Indexes (AIs) were determined by terminal-deoxynucleotidyl Transferase Mediated Nick End Labeling (TUNEL) staining. TNF-α levels were lower in the experimental group compared to the control group (p<0.05) while there was no significant difference in IL-1β level between the 2 groups. MDA levels in the experimental group were significantly lower than in the control group at 2 and 24 h after reperfusion (p<0.05) SOD levels between the groups were not different. The AI of hepatic cells in the control group was significantly higher than in the experimental group (p<0.05). Liver mitochondria of the control group were in disorder and apoptotic bodies had formed; these phenomena were not found in the experimental group. Recombinant growth hormone preconditioning can protect against ischemia-reperfusion injury in rat liver.
Zhao Li, Jie Gao, Pengji Gao, Wenxia Li and Jiye Zhu, 2013. Effects of Recombinant Growth Hormone Preconditioning on Ischemia-Reperfusion Injury in Rat Liver. Journal of Animal and Veterinary Advances, 12: 1415-1419.