Abstract: To induce Pancreatic Duct-derived Stem Cells (PDSCs) of rats into insulin-secreting cells and to determine the in vitro and in vivo function of the insulin-secreting cells. PDSCs were obtained through in situ collagenase digestion of rat panrease and in vitro adherent cultivation, with the phenotype identified and then PDSCs were induced into Islet-like Cell Clusters (ICCs) through serum-free culture with their response to glucose challenge determined. Furthermore, ICCs were transplanted under the kidney capsule of diabetic nudes, with their blood sugar level monitored to determine the in vivo function of ICCs on regulating blood sugar. PDSCs of rats resembled Mesenchymal Stem Cells (MSCs) with CD34 and CD45 positive and CD90 and CD105 negative on phenotype, simultaneously with the expression of PDX-1 and nestin. PDSCs could differentiated into insulin-secreting ICCs through serum-free cultivation. After transplantation under the kidney capsule, ICCs could just softly down-regulated the blood sugar level of diabetic recepient nudes, showing the immaturity of ICCs. PDSCs of rats resembled MSCs, with the expression of PDX-1 and nestin which could differentiated into insulin-secreting ICCs in vitro and down-regulated the blood sugar level of diabetic recepient nudes after transplantation.
Bo Jin, Yan-Ping Liu and Hai-Jiang Wang, 2013. Differentiation of Pancreatic Duct-Derived Stem Cells of Rats into Insulin-Secreting Cells with Function of Down-Regulating Blood Sugar Level after Transplantation. Journal of Animal and Veterinary Advances, 12: 1565-1573.