Abstract: The treatment of bone defect is the multi-disciplinary clinical problem. Although, several types of bone graft materials are used for clinical study, numerous hurdles such as infection and immune rejection need to be resolved in essence. Various types of stem cells combining with biological material for repairing bone defect is a promising way. However, the poor resource of stem cells has restricted their clinical application. In earlier research, researchers had reported that transient in vitro epigenetic reprogramming of mouse skin fibroblasts into multipotent stem cells. In this study, researchers used the similar method to reprogram dog skin fibroblasts into multipotent stem cells. Further, reprogrammed cells were re-differentiated into osteogenesis-like cells in vitro. Especially, iMS cells combining with Hydroxyapatite/β-Tricalcium Phosphate/Chitosan (HAP/β-TCP/CS) Composite Scaffold could repair bone defect in dog. The results indicated that the iMS could be used as seed cell for repairing bone defect in clinical application.
Xiang-Qing Zhu, Li Lei , Qiong Wu , Qiang Wang , Cui-Zhu Chen , Xue-Min Cai , Rong-Qing Pang , Qing-Hua Chen and Xing-Hua Pan , 2013. Induced Multipotent Stem Cells Combining with HAP/TCP/CS Composite Scaffold Repairs Bone Defect. Journal of Animal and Veterinary Advances, 12: 573-580.