Authors : Guangping Gao, Guisheng Gao , Qiumei Shi, Yanying Zhang , Donglin Zhang and Hong Yan Ding
Abstract: To explore the pathogenesis of causeing diarrhea in mink by E. coli, researchers used PCR to detect the pathogenicity island genes LEE (Ler, eaeA) and HPI (irp, fyuA) of three E. coli whose isolation and identification of serotypes are O38, O78 and O29. Researchers designed four pairs of specific primers according to its gene sequence. The genomic DNA as the template for amplification. Results of the three E. coli are that irp2 gene and fyuA gene of HPI were success fully amplified but the LEE (Ler, eaeA) genes were not amplified. Sequence of HPI (irp2, fyuA) showed that the homology about irp2 of the three E. coli is between 98.5 and 99.3%. The homology with reference E. colis irp2 gene is between 98.5 and 99.3%.The homology about fyuA of the three E. coli is between 96.7 and 97.4%. The homology with reference E. colis fyuA gene is between 95.8 and 99.4%.
Guangping Gao, Guisheng Gao , Qiumei Shi, Yanying Zhang , Donglin Zhang and Hong Yan Ding , 2013. Detections of Genes Associated with LEE and HPI Pathogenicity Island Which are Comes from Pathogenic E. coli of Minks. Journal of Animal and Veterinary Advances, 12: 655-659.