Abstract: The effects of β-carotene on differentiation of Bovine Intramuscular Preadipocytes (BIP) and Subcutaneous Preadipocytes (BSP) via BCO2 and related genes were studied. After treatment of cells with 0, 10, 20, 30, 50 and 80 μM β-carotene, cellular lipid content was assessed by Oil Red O staining and a Triglyceride (TG) assay. The relative expression of β-Carotene-15, 15'-Momoxygenase 1 (BCMO1), β-Carotene-9', 10'-momoxygenase2 (BCO2), Peroxisome Proliferator-Activated Receptor-γ (PPARγ) and Retinoic acid X Receptor α (RXRα) were also investigated. Results showed that β-carotene can suppress adipocytes differentiation as a whole. And different concentration β-carotene addition to BSP had not obvious variation of BCO2, PPARγ and RXRα while BIP of same treatment had a highest expression of 30 μM β-carotene at 5 h which can be showed through the results of both Oil Red O staining of cells and fluorescence RT-qPCR. Besides, researchers found that BCMO1 did not nearly express in adipocytes of bovine but BCO2 had a good expression. The present study provides further evidence of the effects of β-carotene on lipid metabolism and suggests that BCO2 may play an important role in the development of bovine adipose tissue in vivo.
Qiaoli Wei, Xiaomu Liu, Fachun Wan, Zhonghua Wang and Xueyan Lin, 2014. Effect of β-Carotene on Differential Gene Expression of BCO2 and Related Genes During Bovine Adipocytes Differentiation. Journal of Animal and Veterinary Advances, 13: 290-296.