Authors : Wang Huishu, Zhang Shiduo, Xu Shiyuan and Zhang Liangcheng
Abstract: To investigate the efficacy and mechanism of dexmedetomidine on myocardial apoptosis induced by grave scalding rat model. Grave scalding rat model was established. The 50 SD rats were randomly divided into five groups (n = 10): normal control (group C), simple scald group (group B), scald and 50 μg kg-1 dexmedetomidine group (group D1), scald and 30 μg kg-1 dexmedetomidine group (group D2), scald and 10 μg kg-1 dexmedetomidine group (group D3). Blood presure and heart rate were atraumatic measured at 0, 3, 6, 9 and 12 h, the blood and the myocardial tissue from ventriculus sinister were harvested which were assayed the troponin, myocardial apoptosis and the expression of caspase-12, GRP78 and CHOP by immunohistochemistry, terminal deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) and Western blotting. The HR (Heart Rate) was various in the five groups which was significant difference (p<0.05). The number of buffy-stained nucleolus in the myocardial cell was the most in the simple scald group. The number of buffy-stained nucleolus of myocardial cells in the D1 and D3 group were more than that in the D2 group which were more than in the normal control group. The expression of caspase-12 obviously increased in the simple scald group compared with that in the normal control. The amount of caspase-12 was the most in the simple scald group. The expression of caspase-12 obviously decreased in the D2 group compared with that in the D1 and D3 group. The proteinic expression of GRP78 and CHOP increased in the B and D1 group which were higher than that in the normal control (p<0.05). The proteinic expression of GRP78 and CHOP in the D2 and D3 groups were less than that in the B group (p<0.01). The proteinic expression of GRP78 in the D2 group was less than that in the D3 group (p<0.05). The proteinic expression of CHOP in the D2 and D3 groups were more than in the normal control (p<0.05). The 30 μg kg-1 dexmedetomidine could lessen the myocardial cellular apoptosis induced by scald, inhibit the expression of caspase-12, GRP78 and CHOP which may be the optimal option for the prevention and therapy of the shock heart compared with 10 and 50 μg kg-1 dexmedetomidine.
Wang Huishu, Zhang Shiduo, Xu Shiyuan and Zhang Liangcheng, 2014. The Efficacy and Mechanism of Dexmedetomidine on the Myocardial Apoptosis Induced by Grave Scalding Rat Model. Journal of Animal and Veterinary Advances, 13: 377-384.