Authors : Hongbin Pan, Sumei Zhao, Jiawei Jiang, Ying Huang, Minghua Yang, Yongke Sun, Shizheng Gao and Guixin Qin
Abstract: Fat-Specific Protein 27 (FSP27) could be a potential biomarker promoting neutral lipid storage and thereby a candidate gene for the regulation of the Intramuscular Fat (IMF) deposition in pigs. In this study, the cloning and comparison of the Coding Domain Sequence (CDS) and the deduction of amino acids sequence of FSP27 gene in longissimus dorsi muscle between Wujin and Landrace pigs suggest that the CDS of FSP27 gene is 747 bp encoding for 248 amino acids. One silent polymorphism (C→T) occurred in 549th nucleotide in the CDS of the FSP27 gene in Wujin pigs. Secondary structure analysis of FSP27 deduced amino acids found 67 helixes, 49 strands, 132 coils and 8 transmembrane helices. The FSP27 was localized in the mitochondria. Moreover, The FSP27 contained 6 exons which the sequence lengths was 33, 78, 154, 159, 188 and 682 bp, respectively. The 3'-UTR region of FSP27 gene could to contain potential regulatory sequences for some miRNA for example hsa-miR-491-5p and hsa-miR-4510. The promoter region of FSP27 gene exhibited binding sites of AML-1a, NF-κ-β and SRY transcription factors. The expression level of the FSP27 mRNA was significantly higher in longissimus dorsi muscle of Wujin pigs than Landrace pigs (p<0.05). The different expression levels of the FSP27 mRNA in longissimus dorsi muscle of pigs may relate to the variation of the IMF eposition.
Hongbin Pan, Sumei Zhao, Jiawei Jiang, Ying Huang, Minghua Yang, Yongke Sun, Shizheng Gao and Guixin Qin, 2014. Molecular Characterization and Tissue Expression of the FSP27 Gene in Wujin Pigs. Journal of Animal and Veterinary Advances, 13: 401-409.