Authors : Alaa. H. Sadoon, Xiwang Liu and Jiyu Zhang
Abstract: Cynanchum komarovii Al. Iljinski is a plant belonging to Asclepiadaceae family, distributes widely in the grassland of Northern China and has been used for preventing and reducing inflammation as a traditional Chinese medicine. The phytochemical screening for C. komarovii plants revealed a number of bioactive constituents including alkaloids, flavonoids, polysaccharide, terpenes, fatty acid, phospholipids, amino acids and vitamin. Researchers have been identified thirteen types of alkaloids from C. komarovii. Therefore, the present study aimed for investigating quantity of alkaloids in these plants and best methods of extracting crude alkaloids from these plants. Three methods was used for extracting alkaloids from C. komarovii plants, the first methods depended on soaking the plants in 95% alcohol and water, evaporating out the alcohol and adding HCL. The second method depend on soaking the plants in 95% alcohol without water and the third method was depend on soaking the plants in 3 times 95% industrial alcohol and 1 L water that contain 10 mL of HCl and leaves at room temperatures for 12 h. Results of extraction of alkaloids from C. komarovii plants revealed the best methods for extraction was the third methods and extracting yield by this was 2.3 g alkaloids for each 1 kg of plants and this represent high quantity that researchers reach for it in the study. Extraction total alkaloid from C. komarovii becomes more perfect by mixing acidic solvent with water and alcohol.
Alaa. H. Sadoon, Xiwang Liu and Jiyu Zhang, 2014. Extraction of Alkaloids from C. komarovii Al. Iljinski. Journal of Animal and Veterinary Advances, 13: 905-907.