Authors : A.M. Erfan and Sh. Marouf
Abstract: Surveillance on Staphylococcus aureus in poultry farms and poultry abattoirs in 2015 in Egypt was applied for determining the biofilm formation, an important virulence and antibiotic resistance determinant. Sixty-seven (68.3%) out of 98 poultry and environmental samples collected in this study were positive for S. aureus. For phenotypic analysis of biofilm formation, Microtitre Plate (MTP) and Congo Red Agar (CRA) tests revealed 63 (94.02%) and 59 (88.05%) positive samples, respectively. A high correlation (94.02%) between MTP and CRA tests was encountered. The PCR showed variable results for genes encoding Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) and for those encoding Polysaccharide Intercellular Adhesion/Poly-N-Succinyl-β-1-6-Glucosamine (PIA/PNSG). Partial DNA sequencing of 606 nucleotides of the icaR-icaA fragment in 21 selected strains showed 98.2%-100% identity with two clearly distinct phylogroups. This study indicated that biofilm producing S. aureus are widely distributed in poultry and poultry abattoirs in Egypt.
A.M. Erfan and Sh. Marouf, 2015. Biofilm-Producing Staphylococcus aureus Screening in Poultry Farms and Abattoirs. Journal of Animal and Veterinary Advances, 14: 308-314.