Abstract: The aim of this study to compare THI method using by average, minimum or maximum temperature and humidity and to compare the Holstein (H), Brown Swiss (BS), Simmental (S) and Anatolian Black Cross (ABC) genotypes using in feedlot by ability to tolerate temperature and humidity under climatic conditions of Sanliurfa province of Turkey. Production data set obtained by a commercial farm which consisted 70,594 test day records for 11,117 cattle (6513 Holstein, 3546 Brown Swiss, 838 Simmental and 220 Anatolian Black Crosses). Weather data provided the nearest weather station and 9.04 km away from to feedlot. Using daily maximum, minimum and average air temperature and humidity values, Temperature-humidity Index (THI) values were calculated by three different combinations for each animal. Analyses were based on model that included effects of year, sex, age, season, days on feed, begin to fattening and several types of THI. According to findings, S and ABC genotypes slightly more tolerated the heat stress compared with H and BS. In addition to BCgenotype was more sensitive to cold stress when compared other genotypes. On the other hand using different combination of temperature and humidity variable (max or min) in THI formula obtained different breakpoint values for stress and comfort zone interval. 72 THI values which is a threshold for starts of heat stress obtain using only maximum temperature and minimum humidity variables in this study. Results from this study indicated that trend of temperature and humidity in the air were determinant factors for THI calculation types when used data from the weather stations.