Authors : E. Kabourkova, J. Hulikova and P. Slama
Abstract: The objective of this trial was to study the Inflammatory cytokinesinterferon gamma (IFN-γ) and Transforming Growth Factor Beta1 (TGF-β1) which are produced by bovine mammary gland leukocytes. The leukocytes were obtained by mammary gland lavage and incubated for 1, 2 and 18 h with lipopolysaccharide (50 μg/mL) cor with muramy l dipeptide (500 μg/mL) in vitro. Lipopolyccharide was used as a toxin of Gram-negative bacteria and muramy l dipeptide as a structural unit of a peptidogly can of Gram-positive bacteria. The concentration levels of these cytokines were analyzed by ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher IFN-γ concentration was recorded for lipopolysacharide incubation. The production of IFN-γ started 18 h following the cell incubation with lipopolysaccharide or with muramy l dipeptide. The production of TGF-β1 was higher for lipopolysaccharide incubation than for muramy l dipeptide incubation throughout the all set time points. The TGF-β1 production started 2 h following the cell in cubation with muramy l dipeptide.
E. Kabourkova, J. Hulikova and P. Slama, 2017. Production of Interferon Gamma and Transforming Growth Factor Beta 1 from Bovine Mammary Gland Leukocytes. Journal of Animal and Veterinary Advances, 16: 119-121.