Abstract: The objective of this study was to get a better knowledge of inflammatory cytokine production (TNF-α, IL-4, IL-10 and TGFβ1) throughout inflammatory response of bovine mammary gland caused by bacterial toxin Lipopolysacharide (LPS) and Muramyl Dipeptide as a structural unit of Peptidoglycan (MDP). In the experiments, there were used LPS and MDP at the specified concentrations (5 and 500 μg, respectively). The inflammatory response was analyzed at selected time points (24, 48, 72 and 168 h) following stimulation of the mammary glands. As a control, the same volumes of physiological solution was used. The mammary gland lavages were collected from the 8 clinically healthy virgin Holsteinx Czech Pied heifers and the concentrations of the cytokines were analyzedby an ELISA method.
E. Kabourkova and P. Slama, 2018. Effect of Lipopolysaccharide and Muramyl Dipeptide on the Inflammatory Cytokine Production Throughout Inflammatory Response of Bovine Mammary Gland. Journal of Animal and Veterinary Advances, 17: 100-103.