Authors : Hayder Abdul-Kareem Hasan AL-Mutar
Abstract: In the present study, the investigation ovulation rate and fertility of Iraq goat by BMP-15 gene after estrus synchronization by using 20 mg impregnated sponges with MAP for 12 days with 400 IU PMSG on day before sponges withdrawal. All 25 Iraqi goats showed (100%) estrus after sponge withdrawal. Twinning percentage 57.9% while single percentage 42.1%. The polymorphisms of BMP-15 gene were analyzed as a genetic marker in Iraqi goats have shown that natural mutations in prolific goat breeds in BMP-15 gene mutations are crucial for increasing litter size as well as ovulation rate. FecXR, FecXB and FecXH mutations increased prolificacy in goats, PCR primers were used to polymorphisms in female goats by using PCR-RFLP method. Although, the sequencing data showed four novels polymorphic sites in goat, mutations in base number. 102 G>A, 156 T>G, 207 G>A and 218 G>T were identified, all these mutations were reported and registered in NCBI with Accession No. of JX860305.1. Amino acid change is Arginine>Lysine, Methionine>Arginine, Arginine>Glutamine and Valine>Leucine with Accession No. of AFX69269.1. The conclusion of BMP15 gene may be a major gene which affects the prolificacy in goat and could provide basic molecular data on the reproductive characteristics of Iraq breeds and a scientific basis for the conservation and utilization of goat.
Hayder Abdul-Kareem Hasan AL-Mutar , 2018. Molecular and Physiological Role of BMP 15 Gene Associated with Ovulation Rate and Fertility of Iraq Goat. Journal of Animal and Veterinary Advances, 17: 104-110.