Authors : Chaturong Inpad, Seksit Singjam, Pimthip Thipbanjong, Prachanat Inprom, Gunticha Suwanmanee and Worasak Kaewkong
Abstract: Avian tuberculosis caused by the infection of Mycobacterium avium. The incident of M. avium infection in poultry, especially, domestic duck was reported in the lower Northern Region of Thailand. This bacterial specie can cross-infecting to other animals and more likely to occur in persons with pre-existent diseases and in immune systems impaired-person. Laboratory routine methods for detection of M. avium are acid fast bacilli staining and subsequently culture in specific medium which time-consuming and false negative results were often reported. This study aims to explore the expression profiles and identification of serum proteins that responding to avian tuberculosis in domestic duck. Serum samples of M. avium infected and healthy ducks were analysed by two-dimensional electrophoresis. Hyper-intensity protein spots as estimated as approximately 22, 35 and 40 kDa in M. avium-infected serum were identified by LC-MS/MS. They were identified as Apolipoprotein A-1, transthyretin and immunoglobulin Y heavy chain. These proteins maybe hypothesized-upregulated for responding to M. avium infection and might be serving as alternative biomarkers for further development of screening test kit in poultry serum or secretion especially, for preventing the epidemiology of these pathogens.
Chaturong Inpad, Seksit Singjam, Pimthip Thipbanjong, Prachanat Inprom, Gunticha Suwanmanee and Worasak Kaewkong, 2018. Serum Proteomics Identifies Candidate Proteins Respond to Avian Tuberculosis in Thai Domestic Duck (Anas platyrhynchos Domestica). Journal of Animal and Veterinary Advances, 17: 122-126.