Abstract: The recent study focused on infertile women by whom they subjected in antagonist ICSI protocol without progressions. Three groups were under study: 25 ICSI women by ages 18-42 years: 20 infertile women(positive control): 20 healthy women(negative control), both of two controls were within fertile period. ICSI protocol practiced with a regimen of Intramuscular (IM) or Subcutaneous (SC) hormones injections (FSH,75 IU/day and LH,75 IU/day) at cycle day 2. While Cetrorelix hormone (0.25 mg/day) was subcutaneously injected at cycle day 7-10. Also single (IM) or (SC) injection of HCG (10000 IU) hormone was administered to ICSI women until we obtain mature follicles. Clinical investigations were done for the studied groups at cycle day 2, 7 and 10.The cycle day 2 of menstruation revealed good selection for infertile women then subjected with ICSI protocol according to significant decrease in sera Estradiol (E2) hormone level in which related to suitable level of FSH (6.49 0.45 m IU/mL) with a significant increase in LH level (6.18±0.94 mIU/mL) in p<0.05 and no significant differences in prolactin hormone level. The above equal amount of sera FSH and LH lead to predict for endometriosis or polycystic ovary syndrome which verified by ultra sound examination and by resultant of serum IgG and IgA increment as anti-FSH and anti-LH. ICSI protocol women sera revealed to significant decrease in serum sugar (110.78±3.19 mg/dL) due to administer Glucophage, significant elevation in SGOT (21.53±2.44 U/L), p<0.05 because of treatment and no significantly differences in patients sera for alkaline-phosphatase and creatinine while there was a significant decreased in C-reactive protein, this due to mild ovarian stimulation, no inflammatory response and the patients reached to follicular phase. ICSI protocol women sera revealed no significant differences in their immune-globulins precursors concentrations due to treatment as in sera protein electrophoresis results whereas sera revealed significant decreasing in C-reactive protein concentration 42.20±17.45, p<0.05.The patients were in low immune responding and ought to administer immune-globulins to improve their pregnancy success percentage more than 20%.