Authors : Gomez-Salazar Edgar Omar, Lemus-Flores Clemente, Mejia Martinez Karina, Ruelas-Hernandez Pablo German, Sanchez-Chipres David Roman, Segura-Correa Jose Candelario and Alonso-Morales Rogelio Alejandro
Abstract: The objective was to compare three active immunization schemes using a myostatin P2-P30 recombinant DNA vaccine and myostatin P2-P30 recombinant antigen in two different doses to measure the effect on antibody levels, back fat, body weight up to 84 days and body weight gain. The 36 growing pigs were divided into four groups: a control group without immunization; Group 1 and 2 used a prime-boost immunization system and Group 3 a classic immunization system. The doses of vaccine and antigen were 100 μg for Group 1 and 1000 μg for Group 2 and 3. Antibody levels at doses of 100 μg (0.76 Abs) were not different in unimmunized pigs but were lower and different (p<0.0001) compared to 1000 μg doses of vaccine and antigen (1.26 and 1.17 Abs). The dorsal fat in pigs with the 1000 μg dose was lower (16.72 and 15.22 mm) (p<0.05) compared with pigs in the 100 μg (19.83 mm) dose. Only the 1000 μg dose of the P2-P30 vaccine or recombinant antigen produces an immune response in pigs with more final weight and body weight gain.
Gomez-Salazar Edgar Omar, Lemus-Flores Clemente, Mejia Martinez Karina, Ruelas-Hernandez Pablo German, Sanchez-Chipres David Roman, Segura-Correa Jose Candelario and Alonso-Morales Rogelio Alejandro, 2019. Effect of Prime-Boost Immunization Against Myostatin on Antibody Levels, Backfat and Growth in Pigs. Journal of Animal and Veterinary Advances, 18: 65-70.