Journal of Animal and Veterinary Advances

Year: 2020
Volume: 19
Issue: 8
Page No. 113 - 116

Expression of Ets Transcription Factor Etv5 Subfamily in Mouse Testis

Authors : Wen-Qain Zhu, Lei Si and Xue- Ming Zhang

Abstract: Spermatogenesis is a complicated biological process which involves the comprehensive regulation of the hormones, growth factors and transcription factors. It’s demonstrated that the E26 transformation-specific (Ets) family transcription factor Etv5 (also named as Ets Related Molecule, ERM) plays a critical role in the maintenance of male germline stem cell niche. However, the basic expression levels of Etv5 and its peer member Etv1 (also known as ER81) and Etv4 (also known as Pea3) during the postnatal development and growth of the testis are still unclear. To know this, testicular samples were collected from 5-days-, 7, 16-weeks-and 10-months-old mice. Etv1, Etv4 and Etv5 were cloned using reverse transcription PCR (RT-PCR). Real-time PCR analysis showed that Etv1 remained higher levels at 7-weeks and 10-months stages compared with those of 5-days and 16-weeks stages (p<0.05, respectively). Etv4 expression was very weak at all four stages but also showing an increase tendency at 7-weeks. Etv5 expression was lower at 5-days, extremely increased at 7-weeks (p<0.01) and then dramatically decreased at 16-week and 10-months stages (p<0.01). Collectively, the mRNA expressions of Etv1 and Etv5 in mouse testis reach peak along with the sexual maturity, then decrease and maintain at a relatively high level which putatively suggests Etv1 may has similar function like Etv5 in spermatogenesis.

How to cite this article:

Wen-Qain Zhu, Lei Si and Xue- Ming Zhang, 2020. Expression of Ets Transcription Factor Etv5 Subfamily in Mouse Testis. Journal of Animal and Veterinary Advances, 19: 113-116.

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