Authors : INCE Omer Baris
Abstract: Pestiviruses cause important infections in ruminants worldwide. Pestivirus infections are economically important due to their direct effects on reproduction in the sheep and goat breeding sector. The pestivirus genus of the Flaviviridae family is single-stranded, icosahedral symmetrical, membranous RNA viruses, among which there are also Bovine Viral Diarrhea Virus (BVDV type 1 and 2), Border Disease Virus (BDV) and Classical Swine Fever (CSFV) virus. BDV is the main cause of congenital infections in sheep and it can cause acute, fetal and persistent infections. BDV infections are common in countries where BVDV infections are endemic in cattle worldwide. Clinical disease is rarely reported in goats. Persistently Infected (PI) offspring in goats are less common compared to sheep or cattle. Vertical transmission plays an important role in the epidemiology of the infection. Serological studies have demonstrated that natural pestivirus infections in goats are common in many countries. In this study, 274 blood samples were collected via. random sampling from goats in 34 livestock enterprises in agroecological sub-regions of Denizli Province, since, there is no sufficient information on the seroprevalence of pestiviruses in goats in Denizli Province of Turkey. The samples were centrifuged at 3000 rpm and serum was obtained. Blood serum was inactivated at 56°C for 30 min and then stored at -20°C in a deep freezer until being tested. Serum samples were investigated in terms of the presence of antibodies by the Serum Neutralization Test (SNT) using the BVDV reference strain NADL as a virus strain. The Madin-Darby Bovine Kidney (MDBK) cell culture was used for testing. The infectious power of the BVDV-NADL strain was determined as the Tissue Culture Infective Dose (TCID50/0.1 mL) value of 105. According to the test results, the individual seroprevalence was 10.58% (29/274) and the flock-based seroprevalence was 14.71% (5/34). When the seropositivity rates of the animals were evaluated according to their sex, 12.85% of the female goats and 3.12% of the male goats were found to be antibody-positive. According to the sex of the goats, the seropositivity rates of the female and male animals were found to be statistically significant (χ2:4.90; p:0.02). A significant difference was found in terms of age groups (6-24, 24-48 and 48 months<) (χ2:11.77; p:0.002). As a result, pestivirus infection was detected in goats in livestock enterprises in Denizli Province, although, it was not common. Therefore, large-scale studies are important for the economy of the region and the country to prevent the spread of the infection in question and to determine its prevalence rates.
INCE Omer Baris , 2020. An Investigation of the Seroprevalence of Pestivirus Infection in Goats in Denizli Province of Turkey. Journal of Animal and Veterinary Advances, 19: 144-149.