Abstract: Lumpy Skin Disease (LSD) is a highly infectious disease of cattle caused by a virus belonging to the genus Capripoxvirus of the family Poxviridae. Members of the Capripoxvirus genus are closely related, with genomic identities ranging from 96% between viral species to 99% between isolates of the same species. This study reviews the development and application of available LSD diagnostic methods. These are categorized into several groups and often based on characteristic clinical signs and laboratory diagnosis. Laboratory diagnosis comprises either identification of the virus using electron microscopy, egg inoculation, isolation in cell cultures, fluorescent antibody test or detection of its specific antibody using serological tests. Several Polymerase Chain Reaction (PCR) assays have been developed recently for more accurate and rapid detection of Lumpy skin disease virus in suitable specimens. In most reference laboratories the basic tests for LSD virus diagnosis comprise molecular methods for generic detection of a Capripoxvirus. But these methods do not differentiate between LSD virus, sheep pox virus and goat pox virus. The conventional PCR method is not as fast as real-time PCR for Capripoxvirus but it is reliable and sensitive. Recent advances in diagnostic technology have dramatically altered laboratory testing of LSD virus and the effort to develop efficient and reliable LSD virus detection methods continues now a days. The availability of a cost-effective diagnostic tool for routine determination of Capripoxvirus genotype will assist to clarify the epidemiological picture in the affected regions and effective control of this disease needs sensitive, specific and quick diagnostic tools at each tier of control strategy.