Authors : Jintao Zhang, Junlong Bi, Zhuo Ha, Chao Yang, Caihong Zeng, Xianguo Sun, Xiaoying Yang, Runhuan Yang, Yingbo Lin, Kai- Xing Qu and Gefen Yin
Abstract: Porcine Circovirus 3 (PCV3) infections have been widely diagnosed and impaired the swine farm industry worldwide. The current study carried out molecular detection of PCV3 in 481 clinical samples collected from 44 swine farms with reported reproductive failures in Yunnan province of China during the period of from 2017 to 2018. The overall PCV3 positive rate was 21.4% (103/481) with relatively low positive rate of 11.91% (28/235) in 2017 while a dramatic increase to 30.49% (75/246) in 2018 which implied the expansion of PCV3 in Yunnan. The detection of PCV3 in pigs with no observable clinical symptom indicated the demand of molecular diagnostic method for more effective PCV3 control. Four entire viral genomes and 15 cap genes of PCV3 strains from the positive cases were sequenced. The phylogenetic and population expansion analyses revealed the genetic diversity of PCV3 genome and immunogenic cap gene with no obvious association with geographical origin and pathogenic differentiation. The four Yunnan strains fell into a mini-clade in PCV3b, indicating a novel viral genotype. Our results contributed to better understanding of PCV3 prevalence in Yunnan province, China and provided supporting data for future development of specific and effective PCV3 recombinant vaccine.
Jintao Zhang, Junlong Bi, Zhuo Ha, Chao Yang, Caihong Zeng, Xianguo Sun, Xiaoying Yang, Runhuan Yang, Yingbo Lin, Kai- Xing Qu and Gefen Yin, 2021. Molecular Detection and Genetic Characterization of Porcine Circovirus 3 in Yunnan, China. Journal of Animal and Veterinary Advances, 20: 91-109.