Authors : Nurul Husna Shafie, Syaza Lyana Idris, Nurul Nadiah Hamdan, Saadiah Abu Bakar, Amirah Haziyah Ishak, Nai'mah Isa, Hasnah Bahari and Khairul Kamilah Abdul Kadir
Abstract: The different parts (leaves, stems and roots) of Lasia spinosa in aqueous (hot and cold) and ethanol extracts were analyzed for nutrient composition, antioxidant capacity and enzyme inhibition of pancreatic lipase, α-amylase and α-glucosidase in vitro. Nutrient composition was determined using standard methods. Folin-Ciocalteu and aluminium chloride colorimetric were carried out to determine Total Phenolic Content (TPC) and Total Flavonoid Content (TFC), respectively. Antioxidant activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and β-Carotene Bleaching (BCB) assay. Enzymes inhibition assays were determined using commercial enzyme kits. Proximate analysis of the samples showed content of moisture (78.20, 89.60, 79.09%), ash (0.97, 1.00, 0.97%), protein (1.24, 1.63, 5.33%), fat (0.13, 0.03, 0.07%), dietary fiber (42.40, 52.35, 45.34%), carbohydrate (19.45, 7.65, 14.33%) and energy value (168.77, 142.94, 171.88 kcal/100 g) of leaves, stems and roots, respectively. Results showed that stems contain higher mineral contents compared to leaves and roots. Hot aqueous leaves extracts contained the highest TPC and TFC. The cold aqueous leaves extract possess high antioxidant activities with EC50 value of 312 μg/mL. The highest pancreatic lipase inhibitory effect was the ethanol stems extract. For α-amylase, hot aqueous leaves extract showed the highest inhibition whereas ethanol leaf extracts showed the highest inhibitory effects of α-glucosidase. Lasia spinosa exhibits excellent nutrient composition, antioxidant contents and activities and inhibitory effects against pancreatic lipase, α-amylase and α-glucosidase which imply the inhibition of dietary fat and sugar absorption and thereby might have a great potential in chemoprevention of obesity and diabetes.
Nurul Husna Shafie, Syaza Lyana Idris, Nurul Nadiah Hamdan, Saadiah Abu Bakar, Amirah Haziyah Ishak, Nai'mah Isa, Hasnah Bahari and Khairul Kamilah Abdul Kadir, 2018. Nutritional Composition, Antioxidative and Inhibitory Effects Against Pancreatic Lipase, α-Amylase and α-Glucosidase of Lasia spinosa. Journal of Engineering and Applied Sciences, 13: 8898-8905.