Authors : Ali K. Al-Banaa, Mohammad A. Atiya and Nadhem H. Hayder
Abstract: In this research, a Trickling Packed Bed Reactor (TPBR) is configured to produce biohydrogen (bio-H2) using hydrolysis of an aqueous solution of Waste Active Sludge (WAS) as a substrate in a pilot heterogeneous nanoparticles photocatalysis (UV+TiO2). The microbial community in the reactor system was detected which. The results of COD removal% in the photoreactor in TiO2+UV, UV and dark after 6 h of operation are equal to 73, 55 and -18.4%, respectively with the presence of spore-forming and gram-negative bacteria. Also, the results show that the acid method was better than the heat one in preparing inoculum for TPBR. And the optimum condition for bio-H2 production in TPBR is at HRT 8h and pH 5.5 with the presence of microbial community represented by Citrobacter freundii, Bacillus sp., Clostridium sp., Klebsiella oxytoca, Serratia ficaria and Pseudomonas putida. The bio-H2 volume and COD removal% reach 7.92 mL and 69.55%, respectively in control pretreatment. While Rhizobium radiobacter, Klebsiella pneumoniae sp. pneumoniae, E. coli, Bacillus sp. and Clostridium sp. were indicated in the UV pretreatment that leads to achieving bio-H2 volume and COD removal % of 22.5 mL and 71%, respectively, the microbial community of Klebsiella pneumonia sp. pneumoniae, Burkholderia mallei, Enterococcus columbae, Bacillus sp., Clostridium sp. and Enterobacter aerogenes were indicated in the TiO2+UVpretreatment method with H2volume and COD removal% reaching 39.6 mL and 80%, respectively. The maximum bio-H2 was produced after supplying organic shock rate, becoming 72 mL after 24 h in the batch state.
Ali K. Al-Banaa, Mohammad A. Atiya and Nadhem H. Hayder, 2018. Identification of Microbial Community of WAS in Trickling Packed Bed Reactor Produced Biohydrogen and Studying the Effect of Hydrolysis by TiO2 Photocatalysis. Journal of Engineering and Applied Sciences, 13: 9935-9945.