Abstract: The digestive enzymes in the gut of Parachanna obscura of River Ose, South Western Nigeria was investigated with respect to distribution and activity levels. The result showed that all the enzymes were present at varying levels of activity in the five gut regions namely, stomach, caecae, duodenum, ileum and rectum. Lipases was absent in the ileum and rectum. Lipase activity was highest in the duodenum and minimal in the stomach. Proteases were present in the entire gut. The peak of pepsin activity was found to occur in the stomach while the least occurred in the rectum. Glycosidases (Lactase and Maltase) were found present in the whole gut system while sucrase was absent. Lactase was recorded to have the highest activity in the stomach while it’s least activity was found in the rectum. Peak activities were recorded for pepsin and glycosidases in the stomach while peak activities were recorded for lipases and trypsin in the duodenum. The order of distribution and relative activity of the enzymes in the gut of P. obscura correlate with its nature of food.