Abstract: Vitrification could provide a promising tool for the cryopreservation of fish embryos. However, in order to achieve cryopreservation using vitrification, chilling sensitivity and cryoprotectants toxicity were determined using tench embryos at four developmental stages (eleven, seventeen, twenty three and twenty nine hour). Embryos treated with alcalase (2mL/998 mL, 2min at 22°C) were exposed to chilling with/without warming. Other embryos were exposed to methanol and glycerol at the concentration of 10 and 20% for periods of 20 min. At last embryos were incubated at special incubator cages where hatching rates were counted. Regarding chilling sensitivity, the hatching rates of embryos decreased significantly (p< 0.001) after exposure to 0°C at all developmental stage except 29-h stage compared with the controls. The same results were reported after exposure to chilling followed by warming. The embryo stage most sensitive to chilling was 11-h stage. The 29-h stage exhibited the least sensitivity to low temperature while 17-h and 23-h stages were intermediate in their sensitivity to chilling. The toxicity of methanol increased significantly (p< 0.001) with developmental stage for 11-h, 17-h and 23-h stages. The largest change was a reduction in the toxicity of methanol in 29-h embryos. The highest hatching rates of tench embryos were obtained with 29-h embryos using various concentrations of methanol. The survival trends of different stages of tench embryos exposed to glycerol concentrations were approximately similar to those embryos exposed to methanol concentrations except for 11-h embryos which showed no hatching with both glycerol concentrations. Concerning the viability of embryos after vitrification, unfortunately, we could not obtain viable embryos in any of conditions examined. In conclusion, it was quite difficult to vitrify the tench embryos during this study using various vitrifying solutions and the method reported by Chen and Tian and further studies using other developmental stages, basic, washing and vitrifying solutions are needed to achieve successful cryopreservation.