Authors : Hamad Ashraf , Kokub Rana , Hifzah Zainab and Ikram-ul-Haq
Abstract: The production of alpha amylase was carried out by thermophilic strains of Bacillus licheniformis. The isolated cultures were screened by solid-state fermentation using wheat bran as substrate, moistened with phosphate buffer (pH 7.5). The culture GHB8 that gave the maximum production of alpha amylase (1277 U/g/min) was used for further studies. The maximum production of alpha amylase (1630 U/g/min) was reached when wheat bran was partially replaced with cottonseed meal in the ratio of 3:1 along with 1 per cent starch. The incubation temperature 40oC was found to be optimum for the production of alpha amylase. The enzyme was most active between 60-70?C.
Hamad Ashraf , Kokub Rana , Hifzah Zainab and Ikram-ul-Haq , 2005. Production of Alpha Amylase by a Thermophilic Strain of Bacillus Licheniformis . Journal of Food Technology, 3: 64-67.