Authors : Olubunmi , O. Agarry , Esther , A. Ekundayo , Adekunle and Babatope-Obasa
Abstract: Indicator bacteria of pathogenic and food spoilage capabilities were isolated from grated cassava, starch and flour using MacConkey agar, Nutrient agar, KF agar and Mann Rogosa Sharpe medium. The most frequently encountered bacterial isolates were Bacillus sp. (42.86%). Plasmids DNA were isolated from B. pumilus, B. sphaericus, B. subtilis and Pseudomonas flourescens. The sensitivity patterns of the isolates showed common resistance to Erythromycin, Gentamycin, Cotrimozadole, Amoxycillin and Nalixidic acid among all isolates while P. flourescens and B. subtilis were resistant to Ampiclox. P. flourescens was most resistant to both discs followed by B. pumilus and B. sphaericus. B. substilis was found to be most sensitive to Cotrimidazole (14.0 mm) followed by Ofloxacin (13.0 mm), Tetracycline (12.0 mm) and Amoxycillin and Augmentin (10.0 mm). P. flourescens was found to be most sensitive to Ofloxacin (12.0 mm), Gentamycin and Chloramphenicol (3.0 mm) among the Gram-negative isolates. The resistance of the isolates to most antibiotics probably resulted from the acquisition of resistance plasmids DNA. These plasmids confer both toxigenicity and resistance factor characteristics on the bacteria.
Olubunmi , O. Agarry , Esther , A. Ekundayo , Adekunle and Babatope-Obasa , 2006. Isolation and Antibiotic Susceptibility of Aerobic Bacterial Food Pathogens Associated with Cassava Products. Journal of Food Technology, 4: 156-159.