Abstract: Porcine Pancreatic Lipase (PPL) was used for the hydrolysis of three esters: ethyl butyrate, ethyl acrylate and ethyl acetoacetate, in 25 different organic co-solvents. In this study, we have used a multiple linear regression (stepwise method analysis) in order to interpret the enzyme activity as function of the initial rate of reaction. The selected models, illustrating the enzymatic activity according to several descriptors of organic solvents, show that the activity increases when the solvent polarity decreases. Such correlation between the activity and solvent polarity in terms of partition coefficient (log P), refractive index (nD) and the Hildebrand solubility ( ) is significant when the substrate is more polar and more soluble in water.
F. Benamia , R. Blida , A. Tahar and Z. Djeghaba , 2007. Lipase-Catalyzed Hydrolysis of Esters in Presence of Organic Co-Solvents: A Multiparametric Correlation of the Effect of Co-Solvent on the Enzyme Activity. Research Journal of Applied Sciences, 2: 185-187.