Research Journal of Applied Sciences

Year: 2010
Volume: 5
Issue: 1
Page No. 7 - 12

Changes in Membrane Permeability During Rehydration of Freeze-Dried Weissella paramesenteroides LC11

Authors : A.A. Soro, K.M. Dje and P. Thonart

Abstract: The objective of this study was to assess changes in membrane permeability during rehydration of freeze-dried Weissella paramesenteroides LC11. Viability was assessed using the electrical conductivity measurement (ms/cm/g dry weight) and the plate count method (cfu g-1 dry weight). The symptoms of injury included an increase in the electrolyte leakage during the first 4 h of rehydration in Milli Q water and a decrease in the survival rate, suggesting an increase in membrane permeability during dehydration. An increase in the temperature, NaCl or monosodium glutamate concentration and a decrease in H+ concentration resulted in an increase in the electrolyte leakage and a decrease in the survival rate. However, a decrease in the electrolyte leakage was observed with increasing glycerol, sucrose or maltodextrin and resulted in the maintenance of cell viability. Regression analysis showed that overall, there was a good correlation between the survival rate and electrolyte leakage (R2>0.90). Change in membrane permeability might lead to electrolyte leakage during rehydration and ultimately, cell death.

How to cite this article:

A.A. Soro, K.M. Dje and P. Thonart, 2010. Changes in Membrane Permeability During Rehydration of Freeze-Dried Weissella paramesenteroides LC11. Research Journal of Applied Sciences, 5: 7-12.

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