Authors : Ali Sharifzadeh , Abbas Doosti and Khadijeh Khaksar
Abstract: Brucella sp. and Salmonella abortus ovis are important causes of ovine abortion around the world. Both Bacteria can be serologically diagnosed, but many factors may cause false positive and negative results. Direct methods based on bacteriological isolation are usual, but they are difficult, time consuming and dangerous. Polymerase Chain Reaction (PCR) have been successfully geted usefull details and discribing the detection of Brucella sp. and Salmonella abortus ovis. The detection of these agents in aborted ovine fetuses by multiplex PCR is described. The mPCR was applied to 54 fetal stomach contents. 10 samples collected from ovine fetus were Brucella sp. 24 samples collected were Salmonella abortus ovis. Fourteen samples collected were negative and 6 samples collected were Brucella sp. and Salmonella abortus ovis. Simplicity and the possibility of detection of both bactera in a single tube reaction support the use of the mPCR is the commen method for microbiological diagnosis.
Ali Sharifzadeh , Abbas Doosti and Khadijeh Khaksar , 2008. Simultaneous Detection of Brucella sp. and Salmonella abortus ovis by Multiplex PCR . Research Journal of Biological Sciences, 3: 109-111.