Authors : Morteza Sattari , Habib Zeighami and Shahin Najar Peerayea
Abstract: Chlamydia trachomatis is now one of the prevalent bacteria found in classic Sexually Transmissible Diseases (STD) and as such, constitutes a serious public health problem. Chlamydia trachomatis infections are known to manifest in a variety of syndromes in both men and women when left undiagnosed and untreated. While the clinical presentations in men include urethritis, epididymitis, etc., women suffer more serious complications such as mucopurulent cervicitis, Pelvic Inflammatory Disease (PID), ectopic pregnancy and tubal infertility. It is important that in the most cases infection with these bacteria is asymptomatic. In this study from 145 women with abortion and 75 healthy women (control group), endocervical samples were obtained. After collection of samples and patients records, samples DNA were extracted and PCR test with Chlamydia trachomatis specific primers (KL1, KL2) that based on amplification of 241bp Chlamydia trachomatis fragmant were done and PCR product electrophoresis on agarose gel. From 145 women with abortion in 31 (21.37%) and in healthy women (control group) from 75 women in 3 (4%) Chlamydia trachomatis were detected, which difference is significant (p< 0.05). Women with positive PCR test were in 35-40 age and have a multi abortion. Chlamydia trachomatis one of the bacteria that causes many genital disorders. It is important that, although effect of these bacteria confirm on different genital disease, but mechanism of abortion and infertility that produce is unclear. According the results detection of these bacteria in women with abortion and infertile women can be important.
Morteza Sattari , Habib Zeighami and Shahin Najar Peerayea , 2008. Detection of Chlamydia trachomatis in Endocervical Smears of Women with Abortion . Research Journal of Biological Sciences, 3: 214-216.