Abstract: The aim of the study was to compare, the screening performance of a new liquid-based cytology method, Liquiprep™,with conventional Pap in a low risk population, using colposcopy followed histology as "gold standard". Methods: This prospective study was performed in a general gynecology clinic in ValiAsr University Hospital, Tehran, Iran from February 2004 to March 2005. The split-sample method was used for preparing conventional and liquid-based cytology. A new technique of liquid-based cytology; Liqui-prep™ was used in this study. All positive result of smears and 10% of negative results in each group were submitted to colposcopy and a biopsy taken when any atypical transformation zone was seen. Sensitivity, specificity, positive and negative predictive values and overall accuracy of both conventional and Liquiprep™ methods were computed in relation to histology. A total of 506 patients were analyzed by two cytology methods and in 65 (12.84%) of cases histologic diagnosis was performed. There were more adequate samples with Liquiprep™ (94.7%) than with conventional (92.1%) smears. There was not any LSIL and HSIL report in two groups. ASCUS was diagnosed significantly more with conventional than with Liquiprep™ smear (1.56 vs. 0.79%). Pathologically 50% of ASCUS in Liquiprep™ and 12.5% in CP had squamous abnormality. Liquiprep™ had a significantly higher sensitivity (66 vs. 83%) and specificity (86 vs. 98%) than the conventional Pap smear to detect ASCUS+ at histology. This study confirms the superiority of the Liquiprep™ method to detect cervical lesions.
Nadereh Behtash , Zeinab Nazari , Mahmod Khaniki , Kazem Zendedel , Fereshteh Fakor and Mamak Shariat , 2008. Liqui Prep™ a New Liquid Based Cervical Cytology Method in Comparison with Conventional Pap Smear in Developing Counteries. Research Journal of Biological Sciences, 3: 627-630.