Research Journal of Biological Sciences

Year: 2008
Volume: 3
Issue: 1
Page No. 68 - 73

Cold Pretreatment-Induced Changes in Antioxidant Enzyme Activities and Relative Water Content and Soluble Sugars in Shoots and Roots of Soybean Seedlings

Authors : L.Z. Yadeghari , R. Heidari and J. Carapetian

Abstract: Soybean (Glycine max) is a tropical crop, but is also grown in temperate regions in middle spring to late summer. This crop has an important role in human diet. Cold temperature damage is a common problem for this plant in temperate regions. Physiological responses to chilling, including antioxidative enzyme activity, Relative Water Content (RWC) and soluble sugar contents were investigated in soybean to identify mechanisms of chilling tolerance. Plants were exposed to 15C (cold-acclimated) or 25C (nonacclimated) for 24 h, under 250 mol m-2s-1 Photosynthetically Active Radiations (PAR). Then all plants were exposed to 4C (chilling temperature) for 24h and allowed to recover at 25C for 24 h. We analyzed the activity of Ascorbate Peroxidase (APX), Catalase (CAT) and Guaiacol Peroxidase (GPX) and soluble sugar content and RWC in both shoots and roots of soybean seedlings. It revealed that the activity of APX and CAT and GPX induced in leaves and roots. Increased activity in roots is important for cold tolerance as compared to shoots. The amount of RWC decreased in both roots and shoots, but soluble sugar content increased, especially in shoots as compared to control plants. Chilling sensitive soybean plants can be made tolerant to cold by cold acclimation.

How to cite this article:

L.Z. Yadeghari , R. Heidari and J. Carapetian , 2008. Cold Pretreatment-Induced Changes in Antioxidant Enzyme Activities and Relative Water Content and Soluble Sugars in Shoots and Roots of Soybean Seedlings . Research Journal of Biological Sciences, 3: 68-73.

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