Research Journal of Biological Sciences

Year: 2008
Volume: 3
Issue: 7
Page No. 737 - 741

Identification of Fusarium solani F. sp. pisi the Cause of Root Rot in Chickpea and Assessment of its Genetic Diversity Using AFLP in Northeast Iran

Authors : F. Hasanzade , M. Falahati Rastegar , B. Jafarpour and M. Kermani

Abstract: Twenty isolates of Fusarium solani f. sp. pisi were obtained from diseased chickpea plants collected from different chickpea growing areas in northeast Iran. A pathogenicity test was performed for all isolates. Result indicated that the Fsp. isolates differed in their virulence on the susceptible cultivar Jam. AFLP was used to evaluate the genetic variation among 20 isolates of Fsp. The 4 primer combinations produced 330 scorable bands of which 110 were polymorphic (34%). The dendrogram constructed using UPGMA method, distinguished 4 main groups at 65% similarity level that multi dimensional analysis confirmed it. No clear trend was detected between clustering in the AFLP dendrogram and geographic origin. The AFLP analysis in this present study showed an apparent link with result of pathogenicity tests.

How to cite this article:

F. Hasanzade , M. Falahati Rastegar , B. Jafarpour and M. Kermani , 2008. Identification of Fusarium solani F. sp. pisi the Cause of Root Rot in Chickpea and Assessment of its Genetic Diversity Using AFLP in Northeast Iran. Research Journal of Biological Sciences, 3: 737-741.

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