Authors : H.A. Al-Domi, Z.W. Jaradat, R.M. Jones and Q.O. Ababneh
Abstract: The objective of this study was to examine the effect of household Microwave Heating (MWH) on the immunogenicity of a putative diabetogenic protein among young Jordanian diabetic children. Bovine serum albumin (1 mg mL-1) was modified at physiological pH (7.4) by incubating with equal volume of bovine insulin (1 mg mL-1) at 37°C for 24 h. The developed modified Bovine Serum Albumin with Bovine Insulin (mBI-BSA) was subjected to MWH for 30, 60, 90 and 120 sec, cooled immediately to 0°C prior to analysis. Blood samples were collected from 76 diabetic and healthy children. Sera were tested for presence of antibodies against mBI-BSA by ELISA. Anti-BSA, anti-mBI-BSA and anti-microwave heated mBI-BSA antibodies were detected in diabetic and healthy children. The difference between groups was insignificant (p<0.05). Anti-mBI-BSA antibodies were significantly higher than that produced against native BSA (p>0.05) with no significant difference between groups. The difference in sera and saliva titers against mBI-BSA between diabetic children and their control was insignificant (p>0.05).The effect of MWH with different exposure times on anti-mBI-BSA was nearly similar to that produced against un heated mBI with no significant difference between groups.
H.A. Al-Domi, Z.W. Jaradat, R.M. Jones and Q.O. Ababneh, 2010. Investigating Immune Responses to Putative Diabetogenic Modified Bovine Proteins Subjected to Microwave Heating among Young Jordanian Children with Type 1 Diabetes Mellitus. Research Journal of Biological Sciences, 5: 521-528.