Authors : S. Moshkelani, S. Rabiee and M. Javaheri-Koupaei
Abstract: Analysis of microsatellite markers as useful polymorphic genetic variations helps to characterize different animal species and breeds, moreover with develop molecular genetics techniques, beings identification and parentage testing is possible using a lot of molecular markers. So in the present study, the researchers examine the 14 genetic markers AHT5, AHT4, ASB23, ASB17, ASB2, VHL20, CA425, HMS7, HMS6, HMS3, HMS1, HTG4, HTG10 and LEX3 of Iranian Arab horses and use they in identity testing it species in Iran. To achieve this goal, DNA was extracted from blood samples collected from 13 families of Iranian Arab strain and then the multiplex polymerase chain reaction was used for amplification of fourteen markers with the specific primers and the PCR products were resolved on a non-denaturing 10% polyacrylamide gel by electrophoresis. The PCR products also remaining obtainable with formamide and electrophoresis was carried out on an ABI PRISM 3100 genetic analyzer using the recommended protocols. The average of heterozygocity was 0.656 and the expected of heterozygocity at this population was 0.697. Consequently, it seems that these fourteen markers can be used as an applicable marker for identifying Arabian horse.
S. Moshkelani, S. Rabiee and M. Javaheri-Koupaei , 2011. DNA Fingerprinting of Iranian Arab Horse Using Fourteen Microsatellites Marker. Research Journal of Biological Sciences, 6: 402-405.