Abstract: Retained placenta is defined as the failure to pass all or part of the placenta from the uterus within 24 h of calving. There are several potential causes for placental retention but the effects on the general health of the cow and her subsequent reproductive performance are costly events to the dairyman. In this study which carried out in Tabriz suburb, we used of 30 Holstein cows allocated in the 3 groups and treated as group 1; handling, group 2; oxytetracycline and group 3; concomitant use of method 1 and 2. Based on data revealed that there is a significant difference among group 2 with two other groups. Thus can be state that Method No. 2 is a good way to treatment of retained placenta. The negative effect of retained placentas on subsequent fertility is commonly due to delayed involution of the uterus and chronic endometritis, one of the more common causes of infertility. Some cows with retention are affected with permanent sterility due to pyometra, perimetritis, salpingitis (inflammation of oviducts), ovaritis or severe damage to the endometrium. In most animals, the major economic loss is due to slight to moderate loss of milk and impaired involution of the uterus, thus a delay in conception. Thus, retained placenta must be treated as soon as possible and based on the data oxytetracycline is appropriate method for treatment of this disorder.