Authors : Bahareh Tabarsi, Ebrahim Zabihi, Amrollah Mostafazadeh, Azadeh Fazli, Laleh Alim Marvasti and Saeed Asgary
Abstract: This study evaluated the effect of two endodontic biomaterials on Transforming Growth Factor (TGF)-β1 and Bone Morphogenetic Protein (BMP)-2 levels produced by cultured human osteosarcoma cell line (Saos-2) and the cytotoxicity of these materials. Mineral Ttrioxide Aggregates (MTA) and Calcium Enriched Mixture (CEM) were prepared and extracted by immersing in 1 mL complete medium (RPMI-1640/10%FBS) for 24 or 72 h. Exactly x0.25, 0.5 and 1.0 dilutions of each extract and control were added onto cultured Saos-2. Cell viability was determined using MTT assay and TGF-β1/BMP-2 secretion in the supernatant was measured by ELISA assay. After 24 h, all dilutions showed no cytotoxicity, however the 72 h extract showed cytotoxicity. The secreted BMP-2 concentration by both biomaterials at x0.5 dilutions were higher than the control (p<0.05). Researchers can conclude the cytokine inducing properties of MTA and CEM were comparable as well as their cytotoxicity. Moreover, their extracts could possibly induce BMP-2 secretion. This phenomenon might allow a partial reversal of the condition to pre-disease status one of the main goals in medicine.
Bahareh Tabarsi, Ebrahim Zabihi, Amrollah Mostafazadeh, Azadeh Fazli, Laleh Alim Marvasti and Saeed Asgary, 2012. Cytokine Release of Two Endodontic Biomaterials. Research Journal of Biological Sciences, 7: 260-264.