Authors : Zambou Ngoufack Francois, Kaktcham Pierre Marie, Tiogo Azemfack Huguette Noelle, Guetiya Wadoum Raoul Emeric and Fonteh Anyangwe Florence
Abstract: The present investigation reports the characterization of the bacteriocin produced by the probiotic strain Lactobacillus plantarum 29V isolated from raw cow milk in the Western’s highlands of Cameroon as well as the viability of this strain in the palm kernel oil. The antimicrobial compound synthesized by Lactobacillus plantarum 29V was sensitive to some proteolytic enzymes. It showed remarkable stability at high temperatures and in the presence of organic solvents, detergents and surfactants. It was also active in pH 2.0-10 and NaCl range of 1-7%. The neutralized cell-free supernatant of this bacterium inhibited the growth of several Lactobacillus sp., pathogenic and food spoilage microorganisms. The results of this study showed that palm kernel oil maintains the viable cell numbers of the probiotic strain Lactobacillus plantarum 29V, without any changes of peroxide and acid indexes of palm kernel oil.
Zambou Ngoufack Francois, Kaktcham Pierre Marie, Tiogo Azemfack Huguette Noelle, Guetiya Wadoum Raoul Emeric and Fonteh Anyangwe Florence, 2013. Characterization of a Broad-Spectrum Bacteriocin Produced by a Probiotic Lactobacillus plantarum and Assesment of the Strain’s Stability in Palm Kernel Oil. Research Journal of Biological Sciences, 8: 35-41.