Abstract: About 40% of worldwide identified soft corals are native to the Red Sea. They represent a source of compounds with potential pharmaceutical applications. Soft coral samples collected from Jordanian coast of Gulf of Aqaba were identified morphologically and anatomically and found to be Sinularia polydactyla, S. grayi, S. copressa and Sarcophyton sp. They were extracted with ethyl acetate and the resulting crude extracts were partially purified on lsilica gel column producing fractions with antimicrobial and/or cytotoxic activities. Gram positive Bacillus subtilis was the only inhibited test bacterial strain and Jurkat was the most affected cell line. Crude extract of S. compressa contains the most potent antibacterial compounds with MIC (8-62.5 μg mL^-1) and antitumor metabolites with IC (10-30 μg mL^-1). These activities are due to presence of 50 compounds with different polarities.