Abstract: Marshmallow is a herbaceous perennial plant with many therapeutic uses in herbal medicine. In this research, effects of hydroalcoholic extract of marshmallow's root were studied on electrophoresis pattern of blood proteins. About 50 female mice were divided in 5 groups including control, placebo and 3 treatment groups. Hydro alcoholic extract (50, 100 and 200 mg kg^-1) was injected in peritoneum of mice every other day for 20 days. Blood samples were taken for studying electrophoresis pattern and immunity. Obtained data were analyzed using SPSS program at 5% probability level. According to results, beta globulin concentration was not affected by treatments. Albumin concentration was reduced in 50 and 100 mg kg^-1 groups and the ratio of albumin to globulin was decreased in all experimental groups significantly. Gama globulin concentration of all experimental groups and α-1 globulin concentration of 100 mg kg^-1 were increased significantly. Concentration of α-2 globulin was increased in 50 and 100 mg kg^-1 groups significantly.