Authors : Salam Z. Al. Agha, Nehad R. Al. Yazji and Najah N. Sunalla
Abstract: This experiment pertains to the protective role of vitamin E and olive oil against Cadmium (Cd)-induced toxicity on glucose, total cholesterol, triglycerides and bilirubin, enzyme activities of alanine Amino Transaminase (ALT), Aspartate Amino Transaminase (AST) and Alkaline Phosphatase (ALP) and on liver tissue of New Zealand rabbits. New Zealand white male rabbits weighing 800-1000 g were used in the present study. Rabbits divided into 4 groups. The 1st group is the control group and received only deionized water orally for 8 weeks. The 2nd group received a dose of (2 mg kg-1 b.wt.) of CdCl2 daily. The 3rd group received a dose of (150 mg kg-1 b.wt.) vitamin E prior CdCl2 administration daily. The 4th group received a dose of (1 mL kg-1 b.wt.) olive oil prior CdCl2 administration daily. Animals of both control and experimental subgroups were decapitated weekly. Administration of CdCl2 showed highly significant increases in serum glucose, cholesterol, bilirubin, triglyceride concentration and ALT, AST and ALP activities all over the experimental periods (p≤0.01). However, pretreatment with vitamin E and olive oil decreased serum glucose and total cholesterol all over the experimental periods to become near normal. The histopathological alterations were manifested in the liver of the CdCl2 treated rabbits. Hepatocellular damage as congestion in the central and portal veins with dilation of these veins and congestion in sinusoids degenerative hepatocytes showed with cytomegaly clearly observed at 4th week. Also, diffuse Kupffer cells between the degenerative hepatocytes showed obviously in the 1st and 4th weeks. The histopathological studies in the liver of rats also showed that vitamin E and olive oil markedly reduced the toxicity of cadmium and preserved the normal histological architecture of the tissue. The present study suggested that vitamin E and olive oil may be beneficial in ameliorating the cadmium-induced oxidative damage in the liver of rabbits.
Salam Z. Al. Agha, Nehad R. Al. Yazji and Najah N. Sunalla, 2014. Prophylactic Role of Vitamin E and Olive Oil on Some Physiological and Histological Alterations of Male Rabbits Liver Exposed to Cadmium. Research Journal of Biological Sciences, 9: 73-84.