Abstract: Ziziphus Spina-christi is a famous plant in Jordan available in different regions. It is used traditionally for the treatment of several pathologies. The aim of this study is to investigate and compare the in vitro α-glucosidase, α-amylase and inflammation inhibitory activities for different concentration of methanolic and ethanolic leaf extracts of Z. Spina-christi. Fresh leaves of Z. Spina-christi were collected and subjected to methanolic and ethanolic extraction and were investigated for their anti-α-glucosidase, anti-α-amylase and anti-inflammatory activities. Methanolic extract seems to be a strong potent in both enzymes inhibitory potential compared to ethanolic. The calculated IC₅₀ were 8.9 and 305.6 μg/ml against α-glucosidase activity and 39.12 and 318.4 μg/ml against α-amylase activity for methanolic and ethanolic leaf extracts, respectively. Anti-inflammatory activity, using protein denaturation method, showed that methanolic extract exhibited a highest anti inflammatory effect compared with ethanolic extract and the standard. At the concentration of 100 μg/ml, the anti inflammatory effect were 95.3, 25.2 and 20.2% for methanolic extract, ehanolic extract and standard diclofenac sodium, respectively. This study support and agree with the traditional usage of plants of Z. Spina-christi for the control of diabetes and inflammation.